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. 2023 Apr 24;8(8):e167816. doi: 10.1172/jci.insight.167816

Figure 3. LTβR signaling is required for laminin-regulated conduits.

Figure 3

(A) LTβR and Pdpn expression on cultured WT and Lama4-KO FRC primary cells after 4 passages. (B) Summary of LTβR and Pdpn intensity on WT and Lama4-KO FRC primary cells cocultured with DPBS or heterotrimeric laminin α1β1γ1 (laminin 411) proteins. (C) WT and FRC-LTβR-KO mice received 2.5 μg dextran-FITC (40 kDa) s.c. Draining LNs were collected 5 minutes after injection. Whole-mount scanning immunofluorescence images (×20) of 6-μm cryosections stained for Pdpn and Lyve-1. Scale bars: 500 μm. (D) Quantification of dextran-FITC (40 kDa) and Pdpn intensity in LN CR, around HEVs, T zone, medulla, and B follicles. Representative of 3 independent experiments; 3 mice/group, 5 LNs/mouse, 3 sections/LN, 3–5 fields/section. Data presented as mean ± SEM. **P < 0.01; ****P < 0.0001 by 2-way ANOVA with Tukey’s multiple-comparison test (B) or unpaired, 2-tailed Student’s t test for 2-group comparisons (D).