Skip to main content
. 2023 Apr 24;8(8):e167816. doi: 10.1172/jci.insight.167816

Figure 4. FRC Lama4 regulates FRC proliferation and survival.

Figure 4

(A) Fluorescence images (×20) of WT and FRC-Lama4-KO LN cryosections stained for ER-TR7 and Ki67. Ki67 intensity was quantified in the CR, around HEVs, T zone, and medulla. Scale bars: 500 μm (left) and 100 μm (right). (B) Ki67 expression in freshly isolated FRCs from WT and FRC-Lama4-KO LNs. (C) Left: Ki67 intensity in cultured primary WT and Lama4-KO FRCs. Right: Ki67 intensity in primary WT FRCs and Lama4-KO-FRCs cocultured with PBS or heterotrimeric laminin α1β1γ1 (laminin 411) protein. (D) Representative fluorescence images (×20) of LN cryosections from WT and FRC-Lama4-KO mice stained for ER-TR7 and p16. p16 intensity quantification in the CR, around HEVs, T zone, and medulla. Scale bar: 100 μm. (E) Primary FRCs (passage 4) were isolated from WT and FRC-Lama4-KO mice and stained for β-galactosidase 1 day after subculture. Images (×20) quantified for intensity of β-galactosidase. (F) Primary FRCs were isolated from WT and FRC-Lama4-KO mice and stained with Annexin V and 7-AAD. Data in A–F are representative of 3 independent experiments; 3 mice/group, 5 LNs/mouse, 3 sections/LN, 3–5 fields/section. Data are presented as mean ± SEM. **P < 0.01; ***P < 0.001; ****P < 0.0001 by 2-tailed Student’s t test for single variable differences.