Figure 5. Laminins regulate conduit flow and antigen uptake after immunization.

Mice were immunized with s.c. flank injection of IFA/OVA. Five days later, 10 kDa dextran-FITC and 70 kDa rhodamine B–dextran were injected at the tail base. Ninety minutes after dextran injection, inguinal dLNs were harvested for flow analysis. (A) Dextran-10⁺ and -70⁺ cells in dLNs. (B) Dextran-10⁺MHC II⁺ and dextran-10⁺MHC II^– cells. (C) Dextran-10⁺CD11c^loCD11b⁺ and CD11c⁺ myeloid subsets. Representative data of 2 independent experiments, 3 mice/group. Data presented as mean ± SEM. *P < 0.05; **P < 0.01 by 1-way ANOVA with Tukey’s multiple-comparison test.