Figure 2. Heterozygous β-catenin gain of function in Lyz2-Cre reporter mice maintains an enhanced tumor growth and metastatic phenotype in TNBC and melanoma models.

(A) Primary tumor growth of orthotopically implanted E0771.ML-1 tumor cells in Lyz2-Cre^+/+ YFP^+/– Ctnnb1^Ex3Δ/wt and Lyz2-Cre^+/+ YFP^+/– C57BL/6 mice. (B) Bioluminescence of ex vivo–imaged lungs in A. (C) Proportion of myeloid and (D) lymphoid populations from dissociated whole lungs from A, gated as described in Supplemental Figure 2D and labeled as in C. (E) Primary tumor growth of orthotopically implanted B16F10 cells in Lyz2-Cre^+/+ YFP^+/– Ctnnb1^Ex3Δ/wt and Lyz2-Cre^+/+ YFP^+/– C57BL/6 mice. (F) mRNA expression of Pmel at endpoint from digested lungs from E as labeled. (G) Proportion of myeloid and (H) lymphoid populations from dissociated whole lungs from E; data are representative of 2 independent experiments, gated as in Supplemental Figure 2D. (A–H) In all panels the mean ± SEM values are shown and represent 8–15 (A–D), 13–15 (E and F), or 4 (G and H) individual mice. Statistical analysis is based on 2-way ANOVA (A and E) and 2-tailed t tests (B–D and F–H); * = P < 0.05.