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. 2023 Apr 24;8(8):e165419. doi: 10.1172/jci.insight.165419

Figure 4. Immunophenotype of STK11/LKB1 variant human NSCLC tumors inducing cachexia.

Figure 4

(A–M) Tumor, serum, or inguinal white adipose tissue samples at sacrifice from the experiment in Figure 3 were processed for measurement of the levels of tumor-infiltrating myeloid cells (A–E) by FACS analysis (see Supplemental Figure 1 for gating strategy), tumor mRNA levels relative to H1792 parental cohort of the indicated genes normalized to β-actin (F–J), immunoblot analysis of inguinal white adipose tissue using the indicated antibodies (K), or serum cytokine levels (L and M) as described in Methods. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, and ****P < 0.0001 based on 1-way (A–J) or 2-way (L and M) ANOVA followed by either Dunnett’s (A–J) or Bonferroni’s (L and M) multiple-comparison test for significant differences from the H1792ΔSTK11 cohort. WAT, white adipose tissue.