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Immunostaining of human control versus NEC ileum (first 2 rows) and Neonatal-Intestine-on-a-Chip (control) versus NEC-on-a-Chip (last 2 rows) for the goblet cell marker, Muc2 (red; A, B, I, and J), Paneth cell marker lysozyme (Lyz, red; C, D, K, and L), chromogranin A (CHGA, red; E, F, M, and N), and the proliferation marker Ki67 (red; G, H, O, and P). White arrows denote representative areas of positive staining. Scale bars: 50 μm.
