Skip to main content
. 2023 Apr 24;8(8):e146496. doi: 10.1172/jci.insight.146496

Figure 5. NEC-on-a-Chip has an impaired epithelial barrier.

Figure 5

Immunofluorescent staining of tight junction marker ZO-1 (green) in (A) control chips and (B) NEC-on-a-Chip. Nuclei are stained with DAPI (blue). (C) Apparent paracellular permeability (Papp) was assessed at 0 (n = 23), 24 hours (Control n = 12; NEC-on-a-Chip n = 12), 48 hours (Control n = 6; NEC-on-a-Chip n = 6), and 72 hours (Control n = 6; NEC-on-a-Chip n = 6). n = number of chips. Data represent mean ± SEM. ***P < 0.001, ****P < 0.0001 by 2-tailed, unpaired t test. Scale bars: 50 μm.