Fig. 4.

Transcripts from genes located on B. prasinos–duplicated chromosome C04 present reduced poly(A) tail lengths. Poly(A) tail measurement was performed using modified 3′RACE. PCR amplification was performed using primers flanking poly(A) tail (A) or primers anchored in 3′ UTR just before poly(A) tail as internal control (B). Experiments were performed using total RNA from RCC4222 line (control line) and BP37B line (with a duplicated chromosome C04). Illustrations representing PCR amplification are present on the right panel. A black line represents the messenger RNA, and the orange line represents the ligated adapter used for reverse transcription and PCR amplification. FW, Forward primer. RV, reverse primer.