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. 2023 Apr 27;37(6):1298–1310. doi: 10.1038/s41375-023-01905-0

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A AdCAR T cells were continuously stimulated for 21 days with 10 ng/ml αCD33-AM_Fab in the presence of irradiated OCI-AML-3 cells (E:T = 1:4; n = 3–12). OCI-AML-3 cells and AMs were replenished every third day. AdCAR T cells were isolated at the indicated days and cytotoxicity against OCI-AML-3 cells (E:T = 1:1) after 72 h was assessed by flow-cytometry. B Timeline and overview of the continuous and intermittent stimulation of AdCAR T cells co-cultured with OCI-AML-3 cells over 21 days. C Cytotoxicity of AdCAR T cells isolated from co-cultures against OCI-AML-3 cells at the indicated days (n = 3–12; E:T = 1:1; 10 ng/ml αCD33-AM_Fab). D T-cell proliferation expressed as fold change in CD2⁺ cells compared to conditions without AM (n = 5–12). E IL-2 secretion determined by CBA analysis of co-culture supernatants on day 17 (n = 6) and granzyme B expression of CD8⁺ AdCAR T cells isolated on day 17 and transferred to 72 h cytotoxicity assays (n = 5). Data are presented as mean ± SEM. Statistical analysis: paired t-test; ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.