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. 2023 Jun 5;26(6):1032–1041. doi: 10.1038/s41593-023-01316-5

Fig. 6. Psychedelic-induced neuroplasticity depends on TrkB and BDNF, but not 5-HT2A activation.

Fig. 6

a,b, LSD and PSI produce rapid recovery of GFP-tagged TrkB fluorescence after photobleaching, indicating increased TrkB trafficking into dendritic spines (a). This effect is lost in GFP-tagged Y433F TrkB-expressing neurons (b). c, Representative images of spinogenesis experiments. MAP2 in magenta, phalloidin in cyan. Scale bar, 2 µm. d, LSD and PSI induce robust spinogenesis in mature neuronal cultures derived from WT but not Y433F+/− mice after 24 h of LSD treatment. e, TrkB.FC but not M100907 prevents increase in spinogenesis produced by LSD. f, Representative images of dendritogenesis experiments. Scale bar, 50 µm. g,h, LSD and PSI enhance dendritic arbor complexity 72 h after treatment in neuronal cultures of WT but not Y433F+/− mice as measured by Sholl analysis of neurite intersections. i,j, TrkB.FC but not M100907 prevents increase in dendritic arbor complexity produced by LSD. Data shown as mean ± s.e.m., *P < 0.05. NS, not significant. Detailed statistics reported in Supplementary Table 1.