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. 2023 May 11;26(6):1008–1020. doi: 10.1038/s41593-023-01326-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a, Staining of matching sections shows that in Arg1-cKO animals, only few Arg1+microglia remain when compared to Arg1-Control. Quantification of Arg1+microglia from matching sections (n = 3 animals per group). Female, Arg1^Control – Arg1^cKO, P < 0.0001; male, Arg1^Control – Arg1^cKO, P < 0.0001. Scale bar, x = 1000 μm. b, Arg1+microglia co-localize with the marker GALECTIN-3, as inferred by our RNA-Seq analysis. Scale bar, x = 100 μm, z = 10 μm. In Arg1-cKO basal forebrain, the percentage of CLEC7A +/IBA1 + cells is not statistically significant different to the percentage of CLEC7A + /IBA1 + cells in the basal forebrain of Arg1-Control animals. c, Similar for GALECTIN-3 + /IBA1 + cells. d, Mean intensity of the lysosomal marker CD68 in female and male Arg1+microglia in BF. The images in b, c and e are representative of 4 female animals per group. Scale bars, x = 50 μm, z = 5 μm. e, CD68 expression in BF of Arg1-Control and Arg1-cKO basal forebrain. Scale bars, x = 100 μm, z = 5 μm. Data in a-d are represented as mean ± s.e.m. Statistically significant differences were determined by ANOVA (a), Mann-Whitney U (b), or unpaired t tests (c and d). All P-values are two-sided, and P-values for multiple comparisons were corrected using Bonferroni’s method (a). ****P < 0.0001, n.s. indicates not significant.

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