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. 2023 May 29;26(6):983–996. doi: 10.1038/s41593-023-01341-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a) Schematic depiction highlighting the different domains of FL TDP-43 and amino acid sequence of TDP-43 (279–360) core peptide (CP) region. b) Adaption of the SUMO-based strategy for the recombinant expression and purification of CP. c) IMAC chromatogram of His₆-SUMO-TDP-43 (279–360) CP purification. d) SDS-PAGE analysis of His₆-SUMO-TDP-43 (279–360) CP. e) UPLC of His₆-SUMO-TDP-43 (279–360) CP. f) Reverse phase HPLC chromatogram of His₆-SUMO-TDP-43 (279–360) CP after ULP-1 treatment. g) UPLC of TDP-43 (279–360) CP. h and i) ESI-MS analysis of TDP-43 (279-360) CP. The theoretical molecular weight of TDP-43 (279-36) CP is 8041.7 Da.

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