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. 2023 May 29;26(6):983–996. doi: 10.1038/s41593-023-01341-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — a) Representative TEM image (left) and b) raw cryo-EM image (right) of fibrils from CP (scale bars: for TEM is 100 nm and for cryo-EM is 50 nm). c) Fourier shell correlation curves from RELION between the two independently refined half-maps. d-e) Representative 2D class average images of CP segments (d) and large extracted CP segment (e) with visible helical crossover and corresponding half-pitch distance of 249 Å.