Extended Data Fig. 5. α-Syn S129 phosphorylation site mutations do not disrupt rescue of DHPG-induced eCB-LTD.
a, eCB-LTD recorded in GFP- SPNs in Syn-tKO mice. b, c, eCB-LTD recorded from SPNs expressing α-Syn S129A (b) nor S129D (c) mutations. d,e, Summary of EPSC amplitudes. Postsynaptic expression of either S129 mutant α-Syn can rescue eCB-LTD (GFP-, pooled: n = 9 cells / 5 mice, 97.54 ± 2.28%; GFP+, S129A: n = 8 cells / 4 mice, 66.89 ± 5.24%; p = 1.03e-5; GFP+, S129D: n = 10 cells / 4 mice, 73.62 ± 2.89%, p = 1.39e-4). e, Summary of PPRs (S129A baseline: 0.91 ± 0.05; post-DHPG: 1.04 ± 0.06; p = 7.8e-3; S129D baseline: 0.98 ± 0.04; post-DHPG: 1.18 ± 0.06; p = 2.0e-3; GFP- (pooled) baseline: 1.08 ± 0.04; post-DHPG: 1.07 ± 0.04; p = 0.496). Data are mean ± SEM. (d, e) Box plots are depicted as mean (center), first/third quartile (lower/upper box limits), and minima/maxima (bottom/top whiskers). Statistical significance was assessed by two-sided tests, including ANOVA with multiple comparisons (d) and Wilcoxon signed tests (e) (*** p < 0.001; ** p < 0.01; n.s. non-significant).