Extended Data Fig. 5. Aberrant egg-laying in ATB-1 silenced and ATB-2 silenced flies.

a, Average number of cycles per aborted burrowing episode on a 1% agarose substrate. Only flies that exhibited two or more aborted burrowing episodes were considered here and in b. Burrowing episodes are comprised of discrete, rhythmic cycles (see Fig. 4); the cycle count per burrowing episode was highly positively correlated with the duration of the episode (r = 0.91). Here and in b-d, box bounds, 25^th and 75^th percentile; red line, median; whiskers, 5^th and 95^th percentile; o, data from individual flies; +, outliers; **p < 0.01, ***p < 0.001, n.s., p > .05, two-sided Wilcoxon rank sum test followed by Bonferroni correction (Supplementary Table 7). b, Average number of cycles per egg-expulsion burrowing episode on a 1% agarose substrate. c, Average normalized depth of penetration of eggs released on a 1% agarose substrate. Silencing neurons using either ATB-1 or ATB-2 splitGAL4 yielded significant deficits in subterraneous egg deposition (see also Fig. 3j, right panel), implicating a defect in the common set of terminalia bristle-innervating neurons in producing this phenotype. d, Number of eggs released on a 1% agarose substrate in 4 h.