Figure 4. LOXL1-AS1 induced human cardiomyocyte pyroptosis by targeting miR-761 under H/R conditions. (A) Using quantitative real-time polymerase chain reaction to measure the miR-761 level. (B) Using MTT to detect cell viability. (C) Using the Release Assay Kit to test the LDH level. (D) Using enzyme-linked immunosorbent assay to monitor IL-1β and IL-18 levels. (E) Using Western blot to determine ASC, cleaved caspase-1, GSDMD-N, NLRP3, IL-1β, and IL-18 levels. (F) Using flow cytometry to examine caspase-1 activity. (G) Using immunofluorescence to detect NLRP3 expression. Data were presented as mean±standard deviation, and 3 separate experiments were performed in triplicate.

GAPDH = glyceraldehyde-3-phosphate dehydrogenase; GSDMD = gasdermin D; H/R = hypoxia/reoxygenation; IL = interleukin; LDH = lactate dehydrogenase; LOXL1-AS1 = lysyl oxidase like 1 antisense RNA 1; NLRP3 = NOD-like receptor pyrin domain-containing protein 3.

^*p<0.05; ^**p<0.01; ^***p<0.001.