Fig. 6.

Bufotenin increases phosphorylation of phospholamban (PLB) in the heart. Effect of 1 µM bufotenin on serine 16 phosphorylation of phospholamban (PS16-PLB) in (A, C) isolated electrically stimulated left atrium (LA) or spontaneously beating right atrium (RA) and in (B, D) isolated perfused hearts from wild type (WT) and 5-HT₄-transgenic (5-HT₄-TG) mice. Typical Western blots are seen on upper parts (A and B) and bar diagrams below (C and D) summarize the data. Numbers in columns indicate numbers of experiments. *Indicate significant differences versus WT. As a loading control, we assessed the protein expression of calsequestrin (CSQ) by cutting the lanes of the blot and incubating the lower and upper halves with different primary antibodies