Fig. 4.

Effect of glycaemic variability on SILAC-outcome selected protein levels in the hippocampus of Nrf2^{− /−} mice. (a–g). Hippocampal protein levels (ratio of signal intensities to Nrf2^-/- mice at euglycaemia [Nrf2^-/--EE] data) in Nrf2^−/− mice exposed to an acute hypoglycaemic episode from a euglycaemic baseline and returned to euglycaemia or hyperglycaemia: ACADL (a); PSMA2 (b); PSMA3 (c); PSMB7 (d); 6PGD (e); HSP90B (f); and CDC37 (g). (h–j) Representative immunoblots of ACADL, PSMB7 and PSMA2 (h), HSP90B, 6PGD and PSMA3 (i), and CDC37 (j), and their respective GAPDH loading controls. Results represent mean values ± SEM. *p<0.05, **p<0.01 (Kruskal–Wallis one-way ANOVA followed by Dunn’s multiple comparisons test). E, euglycaemia; H, high, hyperglycaemia; L, low, hypoglycaemia