Fig. 6.

Effect of combined treatment of carotenoid (FUCO) with ASA on glutathione (GSH) levels (A) and Intracellular ROS generation in RAW 264.7 cells pre-treated with LPS (B) (Cells treated with H₂O₂ (ROS, 65%) was considered a positive control). Morphological observations of carotenoid (FUCO) and their combination with ASA in RAW 264.7 cells with ethidium bromide and acridine orange staining (C) (arrows indicate early and late apoptotic cells (200 ×). DAPI was used as a nuclear marker). The microscopic field for ethidium bromide and acridine orange staining and DAPI are different with the same cell lineages. Refer to Table 1 and Fig. 1 for abbreviations and treatment doses. The data represent mean ± SD (n = 5). ^a,b,c,dValues not sharing common superscript letters between treatments are significantly different (p < 0.05) as analyzed by one-way ANOVA followed by Tukey's test. Refer to Table 1 and Fig. 1 for abbreviations and treatment doses