Fig. 5. Telomere dysfunction induced by 6-thio-dG treatment decreases lung tumor implantation.

A Representative images (H&E) of lungs showing LLC implantation at different time points in inbred C57BL/6 mice. B Protocol for the induction of Lewis Lung Carcinoma (LLC) in inbred C57BL/6 mice. 1 × 10⁶ Lewis cells suspended in 100 µl of DMEM or equal volume of DMEM (controls) were injected via the tail vein of 10–12 weeks old inbred C57BL/6 male mice on day 0 (D0) and daily intraperitoneal (i.p.) injections of 6-thio-dG (5 mg/kg) or vehicle were administered once the LLC was established (D7-D14), as well as a follow up of survival until sample collection on D14. Kaplan–Meier survival curves (C), representative images of LLC-challenged mice treated with 6-thio-dG vs. control lungs (H&E) (D), and quantification of lung tumor area (E, F) and foci (G). Data are expressed as mean ± SEM (the number of mice is indicated in each case). ***p < 0.001 (Dunn–Sidak test for multiple comparisons).