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. 2023 Jun 7;14(6):350. doi: 10.1038/s41419-023-05877-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Identification of ufmylation sites in RPL10 with different mutants. B The change of RPL10 ufmylation and KLF4 level after the knockdown of endogenous RPL10 and the overexpression of RPL10 mutants in PANC-1 cells. C The change of cell proliferation after the knockdown of endogenous RPL10 and the overexpression of RPL10 mutants in PANC-1 cells. The proliferation rates were detected by CCK-8 method (n = 10). D The change of colony formation of PANC-1 cells after the knockdown of endogenous RPL10 and the overexpression of RPL10 mutants. The colonies were stained with crystal violet and analyzed (n = 3). E The change of sphere formation of PANC-1 cells after the knockdown of endogenous RPL10 and the overexpression of RPL10 mutants. F The populational change of ALDH1⁺ cells and CD44⁺-CD24⁺ in PANC-1 cells after the knockdown of endogenous RPL10 and the overexpression of RPL10 mutants. Cell populations were analyzed by flow cytometry (n = 3). All experiments were independently repeated for 3 times, and the data are expressed as mean ± SD. ^*P < 0.05 indicates statistically significant versus negative control. ^**P < 0.01 indicates statistically very significant versus negative control.