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. 2023 Jun 6;3:54. doi: 10.1038/s43705-023-00265-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Neighbor joining tree of selected OTUs which showed significant log fold changes during pairwise analysis of sample locations. The top tree depicts a pairwise comparison between root tip and bulk soil, middle tree between root base and bulk soil, and the bottom tree depicts the comparison between root tip and root base. The bar chart around the tree corresponds to log fold changes for each OTU in each of the different containers - test tube, pot or EcoFAB. An outward bar away from the tree represents a positive log fold change in the and an inward bar towards the tree represents a negative fold change in the respective OTU. The significant changes are indicated at the bottom of each node with a symbol. No symbol at the bottom of the node means the fold change is not statistically significant. b Clustering of selected OTUs based on pairwise comparison between sampling locations (ignoring containers) reveals three different clusters. Each OTU is colored by the phylum it belongs to.