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. 2023 Apr 20;30(6):1550–1562. doi: 10.1038/s41418-023-01164-7

Fig. 3. TRAF2 negatively regulates the protein levels of p62 and autophagy flux.

Fig. 3

A, B Knockdown or knockout of TRAF2 increases p62 level. C TRAF2 overexpression decreases the p62 level. D Huh7 was transfected with siCtrl and TRAF family siRNA oligos for 48 h, and p62 protein level was analyzed by Western blot. E Correlation of TRAF2 and p62 in HCC tissue microarrays (*p = 0.0063, Pearson’s correlation coefficient). T, tumor tissue. A, adjacent nontumor tissue. F LC3 and p62 protein level in TRAF2-deletion Huh7 cells. G LC3 punctate structures were shown by fluorescence microscopy (left, bar:10 μm), ***p < 0.001 (right, Student’s t-test, two-tailed, mean ± SEM, n = 5).