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. 2023 May 24;15:1156777. doi: 10.3389/fnsyn.2023.1156777

FIGURE 2.

FIGURE 2

Colocalization of GluN3A subunit protein with postsynaptic density (PSD-95) in the hippocampus and quantitation of puncta in the medial entorhinal area (MEA). (A) Immunolabeling of PSD-95 puncta (images in R1C1, R2C1, and R3C1) and GluN3A (top right inset in R1C1) subunit protein (images in R1C2, R2C2, and R3C2) and their colocalization with PSD-95 (merged images in R1C3-C4, R2C3-C4, and R3C3; arrowheads) in the hippocampus (CA1-3; red box in the bottom left inset of image in R1C1) relative to dendrites immunolabeled with MAP2 at the indicated enlargements (lettered boxes in yellow). Nuclei, labeled with DAPI, indicate cell density within the neuropil. (B,C) Histograms of average density, diameter, and area of PSD-95 (B) and GluN3A (C) puncta in the MEA (error bars indicate s.e.m; n, number of puncta measured; N, animals used). Density estimates (B) were only made for PSD-95 from various non-overlapping regions within MEA imaged at differing magnifications. GluN3A subunit protein puncta were on average smaller in diameter and cross-sectional area compared with PSD-95 [hatched red line, (C)], although these differences were not statistically significant, p = 0.21 and 0.24, respectively, nested t-test.