Fig. 6.

Redox treatment of recombinant mutant variants of HisAtPYR1 in which one cysteine has been replaced by serine. Recombinant wild type rAtPYR1 and mutated AtPYR1C30S (C30S), HisAtPYR1C65S (C65S), and HisAtPYR1C77S (C77S) were treated with 2 mM DTT (first 4 lanes in gel on the left) or 2 mM DTT + 4 mM H₂O₂ (lanes 5–8) as described in Materials and methods. Oligomeric and monomeric forms are pointed, as well as molecular weight markers. Pounceau staining is shown to check loading. Gel on the right is an example with an excess of protein for a better visualization of the oligomers, and table presents the relative quantification of the bands in three independent experiments of the H₂O₂-treated proteins.