Fig. 8.

Western blot analysis for in vivo PYR1 detection. Western blot was performed using $\alpha$-PYR1 antibody in WT plants in non reducing, reducing (+DTT), and oxidizing (+H₂O₂) treatments of the leaf extracts. Extracts (lane 2) were incubated with 50 mM DTT (lane 3) or 4 mM H₂O₂ (lane 4). Marker (M) proteins were loaded in lane 1 for molecular weight position. The pattern of PYR1 oligomerization after treatments of the leaf extracts is evidenced by the presence of the different monomeric, dimeric, and oligomeric forms, as pointed at the right side. The asterisk points a very high molecular weight oligomer and ns points a non-specific band.