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. 2023 May 24;14:1172199. doi: 10.3389/fendo.2023.1172199

Figure 3.

Figure 3

Effects of mPTD-BMP7 on ferroptosis in the kidneys of diabetic mice. (A) mRNA expression of ferroptosis-related genes significantly changed in the diabetic kidney, and the changes were abrogated by mPTD-BMP7. (B) Immunohistochemistry showed that damaged tubular cells exhibited increased level of 4-hydroxynonenal (4-HNE) and malondialdehyde (MDA) in the vehicle group, and it was abrogated by mPTD-BMP7 administration. Prussian blue staining revealed iron deposition in the interstitium of diabetic kidney (yellow arrow), and it was attenuated by mPTD-BMP7. Original magnification: x400 (scale bar: 50μm) (C) Lipid oxidation, assessed via MDA levels, was increased in the kidneys of STZ mice, and reduced by treatment with mPTD-BMP7. (D) GSH concentration in the kidneys of STZ mice was decreased, and it was attenuated by treatment with mPTD-BMP7. (*P < 0.05; **P < 0.01; ***P < 0.001 versus control group. ##P < 0.01; ###P < 0.001; versus mPTD-BMP7 group).