Table 2.
Primer sequences used in qPCR analysis of the cell experiment.
| Gene | Species | Accession number | Primer sequences from 5’ to 3’ |
|---|---|---|---|
| Tgfb1 | Rattus norvegicus | NM_021578 | Forward TGAGTGGCTGTCTTTTGACG |
| Reverse TGGGACTGATCCCATTGATT | |||
| Tgfbr1 | Rattus norvegicus | NM_012775 | Forward TGAGTGGCTGTCTTTTGACG |
| Reverse TGGGACTGATCCCATTGATT | |||
| Col1a1 | Rattus norvegicus | NM_053304 | Forward TTCTGAAACCCTCCCCTCTT |
| Reverse CCACCCCAGGGATAAAAACT | |||
| Ccn2 | Rattus norvegicus | NM_022266 | Forward AGAGTGGAGATGCCAGGAGA |
| Reverse CACACACCCAGCTCTTGCTA | |||
| Fn1 | Rattus norvegicus | NM_019143 | Forward GTGGCTGCCTTCAACTTCTC |
| Reverse TTGCAAACCTTCAATGGTCA | |||
| Gpx3 | Rattus norvegicus | NM_022525 | Forward GAGAAGAGCCGAGACAATGG |
| Reverse GAGCCTAAGCCTGAATGCAC | |||
| Gpx4 | Rattus norvegicus | NM_017165 | Forward CCGGCTACAATGTCAGGTTT |
| Reverse ACGCAGCCGTTCTTATCAAT | |||
| Slc7a9 | Rattus norvegicus | NM_053929 | Forward CAGGGGGTGAGTACCCCTAT |
| Reverse TAAAAGGCCGCACACACATA | |||
| Fth1 | Rattus norvegicus | NM_012848 | Forward ATGATGTGGCCCTGAAGAAC |
| Reverse TCATCACGGTCAGGTTTCTG | |||
| Hspb1 | Rattus norvegicus | NM_031970 | Forward CTGGTGTCCTCTTCCCTGTC |
| Reverse GCTCCAGACTGTTCCGACTC | |||
| 18s | Rattus norvegicus | NR_042637 | Forward CAAGTAGGAGAGGAGCGAGC |
| Reverse CATGTCTAAGTACGCACGGC |