Figure EV1. Immunization with radiation‐attenuated Plasmodium falciparum sporozoites (PfSPZ Vac) induces strong antibody responses against the PfCSP C terminus.

1. Representative ELISA curves of serum IgG reactivity against PfCSP‐derived NANP₅ peptide and C‐CSP domain.
2. Flow‐cytometric single‐cell isolation strategy for PfCSP‐reactive memory B cells and plasmablasts from a representative immunized donor.
3. Frequency of PfCSP‐reactive memory B cells (upper panel) and plasmablasts (lower panel) in PBMCs of immunized donors at the indicated time points (III + 14 and III + 35) and non‐immunized donors (Pf non‐exp).
4. Paired Ig heavy and light chain V gene usage in PfCSP‐reactive memory B cells (n = 1,172).
5. Isotype distribution of IGHV3‐21‐ and IGHV3‐33‐encoded mAbs.
6. Representative ELISA curves of mAbs from PfCSP‐reactive memory B cells (black) or positive (red) and negative (green) control mAbs 2A10 (Triller et al, 2017) and mGO53 (Wardemann et al, 2003), respectively, binding to FL‐CSP (left) with corresponding AUC values (right; n = 267).
7. ELISA binding strength of FL‐CSP‐reactive mAbs to LPS, dsDNA or insulin compared to the highly polyreactive mAb ED38 (bright red; Meffre et al, 2004), weakly polyreactive mAb JB40 (dark red; Meffre et al, 2004), and the non‐polyreactive mAb mGO53 (green; Wardemann et al, 2003).
8. ELISA binding strength of FL‐CSP‐reactive mAbs to N‐Junc peptide vs NANP₁₀.

Data information: Data in (C) represent one biological measurement obtained prior to cell sorting. Data in (F and H) indicate means from three independent technical replicates. Data in (G) are representative of two independent technical replicates.