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. 2023 Mar 28;15(6):e17014. doi: 10.15252/emmm.202217014

Figure 1. Effects of short‐term exposure to PM2.5 on cell viability and EGFR signaling in lung cancer cells.

Figure 1

  1. Normal lung cells (IMR90 and MRC5) and lung cancer cells (A549 and H1975) were treated with various concentrations of PM2.5 for 24 h, and cell viability was assessed by a Trypan blue assay. The values are the mean ± SD of three independent experiments.
  2. H1975 and A549 cells were treated with various concentrations of PM2.5 for 24 h, and their cell lysates were analyzed for phosphor‐ERK (pERK), phosphor‐AKT (pAKT), phosphor‐STAT3 (pSTAT3), phosphor‐EGFR (pEGFR), ERK, AKT, STAT3, and EGFR by Western blotting. β‐actin served as the loading control. The results shown are from one of three similar experiments (left panel). The relative expression levels of pEGFR, pAKT, pSTAT3, and pERK were quantified by normalizing with β‐actin and are shown in the right panel. The values are the mean ± SD of three independent experiments. *P < 0.05, as analyzed with one‐sample t‐test and compared with untreated cells.

Source data are available online for this figure.