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. 2023 Mar 28;15(6):e17014. doi: 10.15252/emmm.202217014

Figure 2. Effects of long‐term exposure to PM2.5 on cell proliferation, EGFR activation, and anchorage‐independent growth of lung cancer cells.

Figure 2

  • A, B
    H1975 and A549 cells were treated with PM2.5 at 50 μg/ml for 90 days, and the proliferation of the treated cells was assessed by Trypan blue assays.
  • C, D
    H1975 and A549 cells were exposed to PM2.5 at 50 μg/ml for different lengths of time, and the cell lysates of treated cells were assessed for phosphorylated EGFR (pEGFR) and EGFR by Western blotting. β‐actin served as the loading control.
  • E, F
    H1975 and A549 cells were treated with PM2.5 at 50 μg/ml for 90 days. The anchorage‐independent growth was assessed by a soft agar colony formation assay. The number of colonies was scored, and the data are presented as the relative colony formation ability.

Data information: The data shown are the means ± SDs from three independent experiments. *P < 0.05 and **P < 0.01, compared with untreated control cells. The results shown in (C and D) are from one of three similar experiments. (A and B) P‐values were determined by two‐sample t‐test. (E and F) P‐values were determined by one‐sample t‐test.

Source data are available online for this figure.