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. 2023 Jun 2;16(5):dmm049488. doi: 10.1242/dmm.049488

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Characterization of zebrafish endoglin chromosomal organization, transcripts and expression pattern. (A) Zebrafish endoglin gene exon-intron structure on chromosome 5. Coding exons (black) and non-coding exons (gray) are shown. (B) RT-PCR analysis of endoglin variants. Endoglin long (arrowheads) and short (asterisks) isoform messenger expression in embryo and adult tissue (brain). (C) Protein alignment of zebrafish Endoglin isoforms. Alternative amino acids (aa) of Endoglin short isoform are in bold. Transmembrane aa are in red. (D) Alignment of human, mouse and zebrafish Endoglin short isoform C-terminal region. Identical (black) and similar (gray) aa are shown. TM, transmembrane. (E) RT-PCR analysis of endoglin variants during zebrafish development. Endoglin short isoform (asterisk) and Endoglin long isoform (arrowhead) are shown. hpf, h postfertilization. (F) Left: whole-mount in situ hybridization using endoglin antisense riboprobe on 24 hpf wild-type embryo. Inset: close-up of the trunk region showing differential endoglin expression between the dorsal aorta (DA) and the posterior cardinal vein (PCV). Arrowheads indicate intersegmental vessels. Right: close-up of the head region showing mostly venous endoglin expression. ACeV, anterior cerebral vein; MCeV, middle cerebral vein; PHBC, primordial hindbrain channel; PMBC, primordial midbrain channel; OV, optic vein. Scale bars: 100 µm.