Table 1.
The effect of ME and solvent fractions of the leaves of R. prinoides on castor oil induced diarrhea model in mice.
| Groups | Onset of diarrhea(min.) | Total stool frequency in 4hrs | Total weight of wet diarrhea | % Inhibition of Total wet fecal output(gm) | Weight of watery content of wet stools(gm) | %Inhibition of watery content of wet stool |
|---|---|---|---|---|---|---|
| ME | ||||||
| 2%tween80,10 ml/kg | 79.83 ± 2.78 | 9.17 ± 1.11 | 1.29 ± 0.08 | – | 0.69 ± 0.11 | --- |
| Loperamide, 3 mg/kg | 147.0 ± 2.80*aeh | 1.13 ± 0.21*aeh | 0.56 ± 0.09*ae | 56.59% | 0.14 ± 0.03*aeh | 84.06% |
| ME100 mg/kg | 98.13 ± 1.08 | 1.05 ± 0.32 | 0.94 ± 0.12 | 29.21% | 0.48 ± 0.10 | 45.28% |
| ME200 mg/kg | 116.3 ± 20.73 | 1.57 ± 0.21*a | 0.44 ± 0.11*ae | 49.14% | 0.28 ± 0.05*ae | 66.41% |
| ME400 mg/kg | 137.7 ± 5.77*aeh | 1.17 ± 0.17*aeh | 0.59 ± 0.05*ae | 51.27% | 0.19 ± 0.01*ae | 79.71% |
| Solvent fractions | ||||||
| n-BF 100 mg/kg | 101.20 ± 0.05*a | 1.08 ± 0.21*a | 0.87 ± 0.02*a | 27.02% | 0.43 ± 0.00 | 42.10% |
| n-BF 200 mg/kg | 121.14 ± 10.0.2*abd | 1.11 ± 0.50*ac | 0.59 ± 0.09*ad | 47.31% | 0.27 ± 0.10*ad | 56.50% |
| n-BF 400 mg/kg | 141.2 ± 2.15*acd | 1.02 ± 0.1*acdf | 0.47 ± 0.01*acd | 54.10% | 0.16 ± 0.02*acdf | 79.30% |
| CF 100 mg/kg | 89.09 ± 2.01*a | 1.75 ± 0.20*a | 0.90 ± 0.03*a | 24.41% | 0.41 ± 0.30 | 40.15% |
| CF 200 mg/kg | 111.31 ± 1.51*ad | 1.59 ± 0.10*ad | 0.52 ± 0.02*ad | 46.50% | 0.25 ± 0.00*ad | 54.21% |
| CF 400 mg/kg | 129.5 ± 1.13*acd | 1.12 ± 0.30*ad | 0.51 ± 0.00*adf | 48.40% | 0.21 ± 0.02*adf | 73.50% |
| PtEF 100 mg/kg | 76.34 ± 0.18 | 2.08 ± 0.12 | 0.97 ± 0.10 | 19.20% | 0.61 ± 0.20 | 32.45% |
| PtEF 200 mg/kg | 109.20 ± 1.05 | 2.05 ± 0.50 | 0.94 ± 0.03 | 37.30% | 0.38 ± 0.01 | 44.20% |
| PtEF 400 mg/kg | 122.10 ± 0.87*ad | 1.18±0.17*ad | 0.73 ± 0.00*ad | 42.45% | 0.27 ± 0.00*ad | 58.00% |
| Loperamide, 3 mg/kg | 143.02 ± 1.81*acdf | 1.0 ± 0.02*acd | 0.45 ± 0.05*acd | 57.50% | 0.12 ± 0.03*acdf | 82.30% |
Values are expressed as Mean ± SEM (n = 6 mice in each group) and analyzed by one way ANOVA followed by Post Hoc Tuckey test; a compared with 2% tween 80, b compared with 100 mg/kg n-BF, c compared with 100 mg/kg CF; d compared with 100 mg/kg PtEF; f compared with 200 mg/kg PtEF; e compared with 100 mg/kg MF; h compared with 200 mg/kg MF; * = p˂0.01; ME = absolute methanol crude leave extract of Rhamnus prinoides.