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. Author manuscript; available in PMC: 2023 Dec 5.
Published in final edited form as: Angew Chem Int Ed Engl. 2022 Nov 9;61(49):e202210935. doi: 10.1002/anie.202210935

Figure 3.

Figure 3.

Dynamic intracellular regulation. (a) Zn2+-specific DNAzyme controlled extracellular mitochondrial aggregation and dissociation (group-1: chain-4 modified, group-2: chain-5 modified). (b) Cell images showing the controlled interaction of group-1 and group-2 extracellular mitochondria (1:1). BF: bight-field, AF 488: green fluorescence, TAMRA: red fluorescence, Merge: mixed green and red channel. (c) TEM images of extracellular mitochondria. Left: assembled mitochondria. Right: disassembled mitochondria after adding Zn2+ ions. (d) Internal regulation of intracellular mitochondria interactions by DNAzyme molecular machines (chain-4 and chain-5, delivered by liposomes). (e) Fluorescent images (with squared areas zoomed) showing PANC-1 cells treated with liposomes containing chain-4 and chain-5 for 1 h, followed by observation under different times. BF: bight-field, AF 488: green fluorescence, TAMRA: red fluorescence, Merge: mixed green and red channel. (f) 3D cell images showing assembled mitochondria. (g) TEM images of intracellular mitochondria. Left: assembled mitochondria in PANC-1 cells treated with liposomes containing chain-4 and chain-5. Right: disassembled mitochondria in PANC-1 cells after adding Zn2+ ions.