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. 2023 Jun 7;160(3):253–276. doi: 10.1007/s00418-023-02204-6

Table 3.

Recommended approaches for sharing project outputs. Online citizen science projects for the analysis of biological volumetric data can result in a diverse selection of project outputs that should be openly shared in a timely manner

Project output example Recommendations Possible venues
Raw image data e.g., www.ebi.ac.uk/pdbe/emdb/empiar/entry/10094 Large, complex data sets should be deposited in a public image archive to enable access.

Electron microscopy public image archive (EMPIAR; www.ebi.ac.uk/pdbe/emdb/empiar)

Image Data Resource (IDR, idr.openmicroscopy.org)

BioImage Archive (www.ebi.ac.uk/bioimage-archive)

BioStudies database (www.ebi.ac.uk/biostudies)

Cell centered database (library.ucsd.edu/dc/collection/bb5940732k)

Nanotomy (www.nanotomy.org)

OpenOrganelle (openorganelle.janelia.org)

WormAtlas (www.wormatlas.org/index.html)

Raw contributor data e.g., www.ebi.ac.uk/biostudies/files/S-BSST448/classifications.tar.gz e.g., www.ebi.ac.uk/biostudies/files/S-BSST448/Expert Sensitive fields should be removed from the classification export prior to sharing. Sharing contributions as a spreadsheet rather than binary masks will reduce the digital footprint. A disclaimer should be provided if the data may contain offensive classifications. Relevant data contributed by experts should also be shared.

GitHub (www.github.com)

Biostudies (www.ebi.ac.uk/biostudies)

Aggregated contributor data e.g., www.ebi.ac.uk/biostudies/files/S-BSST448/Aggregations The mode of data aggregation should be carefully communicated in conjunction with aggregated contributor data. Data may be shared within a spreadsheet via a binary mark.

GitHub (www.github.com)

Biostudies (www.ebi.ac.uk/biostudies)

Aggregation code e.g. www.github.com/FrancisCrickInstitute/Etch-a-Cell-Nuclear-Envelope Aggregation algorithms should be shared for re-use. GitHub (www.github.com)
Machine learning code and data e.g. www.github.com/FrancisCrickInstitute/Etch-a-Cell-Nuclear-Envelope e.g. www.ebi.ac.uk/biostudies/files/S-BSST448/Predictions The accessibility of any shared code for the intended end-user should be considered.

GitHub (www.github.com)

BioImage Model Zoo (www.bioimage.io)

ZeroCostDL4Mic (www.github.com/HenriquesLab/ZeroCostDL4Mic)

Model Zoo (www.modelzoo.co)

Jupyter notebooks

Microscopy protocols Version control should be carefully applied to ensure precise information is captured for each iteration of an experiment. Protocols.io (www.protocols.io)