Table 1.
Pluripotency marker expression of 2D and 3D expanded cells
| 2D planar | 3D Suspension | p-value | |
|---|---|---|---|
| Tra-1-60− Tra-1-81− | 3.31% (IQR 0.95%) | 0.00% (IQR 0.00%) | 0.0476 |
| Tra-1-60− Tra-1-81+ | 0.08% (IQR 0.04%) | 0.00% (IQR 0.00%) | 0.0476 |
| Tra-1-60+ Tra-1-81− | 11.05% (IQR 2.83%) | 1.20% (IQR 0.87%) | 0.0952 |
| Tra-1-60+ Tra-1-81+ | 85.57% (IQR 3.75%) | 98.80% (IQR 0.87%) | 0.0952 |
| Oct4− Nanog− Sox2− | 5.47% (IQR 2.21%) | 0.09% (IQR 0.09%) | 0.0476 |
| Oct4− Nanog− Sox2+ | 26.92% (IQR 5.98%) | 1.13% (IQR 0.42%) | 0.0079 |
| Oct4− Nanog+ Sox2− | 0.09% (IQR 0.04%) | 0.01% (IQR 0.01%) | 0.0873 |
| Oct4− Nanog+ Sox2+ | 5.89% (IQR 0.85%) | 3.95% (IQR 1.30%) | 0.3095 |
| Oct4+ Nanog− Sox2− | 0.27% (IQR 0.16%) | 0.01% (IQR 0.01%) | 0.0873 |
| Oct4+ Nanog− Sox2+ | 6.46% (IQR 2.19%) | 0.25% (IQR 0.08%) | 0.0079 |
| Oct4+ Nanog+ Sox2− | 0.32% (IQR 0.15%) | 0.00% (IQR 0.00%) | 0.0476 |
| Oct4+ Nanog+ Sox2+ | 52.45% (IQR 5.61%) | 94.25% (IQR 1.40%) | 0.0079 |
Quantitative pluripotency marker expression on day 5 characterized by flow cytometry of cells expanded in 2D planar and 3D suspension conditions with percent of total cells and p-value