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. 2023 Jun 6;14:151. doi: 10.1186/s13287-023-03385-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Nanotracker and surface marker analysis of EVs pre- and post-nebulisation. BM-EV (A) and UC-EV (B) samples were analysed by MACSPlex Exosome Kit surface marker antibody panel and flow cytometry, with pre- (left side) and post- (right side) nebulisation samples compared by heat map. In nanotracker analysis, total number of particles was established for each EV type pre- and post-nebulisation (C) and in the exosome size range (D). Particle size distributions were also constructed for BM-EV pre- (E) and post- (F) nebulisation and for UC-EV pre- (G) and post- (H) nebulisation. N = 6