Fig. 6.

IV and nebulised EV effects on lung structure in E. coli induced pneumonia. Rats received a bolus of E. coli, 1 h later were administered BM- or UC-EVs by IV or nebulisation route, and at 48 h lungs were sectioned, stained and airspace calculated by stereology. Representative images of vehicle only (A), BM-EV delivered by nebuliser (B), BM-EV delivered IV (C), UC-EV delivered by nebuliser (D), and UC-EV delivered IV (E) are provided. Stereological analysis was used to graph average airspace in all images (F). Arrows indicate alveolar thickening in vehicle animal. Vehicle N = 5, all other groups N = 8. **p0.01, ****p < 0.001 wrt pneumonia with vehicle. ns = no significant different between IV and Neb delivery