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Table 2.
The reaction conditions of RT-qPCR
| Stage | Cycle number | Temperature | Time |
|---|---|---|---|
| Predenaturation | 1 | 95 °C | 300 s |
| Denaturation | 40 | 95 °C | 10 s |
| Annealing | 40 | 60 °C | 30 s |