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[Preprint]. 2023 May 18:2023.05.16.541014. [Version 1] doi: 10.1101/2023.05.16.541014

Figure 1: Data processing schematic.

Figure 1:

A flowchart describing the data processing steps required prior to the manual curation process. RNA is first sequenced using both PacBio and Illumina platforms. PacBio long reads are trimmed and refined using the IsoSeq pipeline, aligned to the reference genome using Minimap2, assembled into non-redundant transcripts using StringTie, and ORFs predicted using TransDecoder. Illumina short reads are trimmed using Fastp, aligned to the reference genome using STAR, and protein-coding genes are predicted using BRAKER.