Extended Data Figure 2. Microendoscopy Calcium imaging in MPN.

a,b Second example mouse with MPN pan-neuronal calcium imaging during social isolation and social reunion, and 3 clusters of neurons displaying distinct activity patterns during reunion. c. Number of activated MPN neurons during social isolation, group housing, restraint stress (1h) and food deprivation (24h), n=3–6. Scale bar, 200μm. d. Average calcium activity of MPN^Mc4r+ neurons that were inhibited during reunion, n=3. e. Calcium imaging of MPN^Isolation neurons in the initial phase of social isolation. Data below represent average activity across 27 neurons pooled from 2 mice. Wilcoxon signed-rank tests were used to estimate statistical significance of enhanced neuronal activity in each time bin (30s) vs the first time bin, *p<0.05, **p<0.01, ***p<0.001.