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[Preprint]. 2023 May 18:2023.05.17.541253. [Version 1] doi: 10.1101/2023.05.17.541253

Figure 1. Impact of C. difficile CD630Δerm sspA and sspB mutations on sporulation and UV resistance.

Figure 1.

A) Day 6 sporulating cultures were fixed in 4% formaldehyde and 2% glutaraldehyde in PBS and imaged on a Leica DM6B microscope. B) Strains were grown on 70:30 sporulation medium for 48 hours and the cultures then were treated with 30% ethanol and plated onto rich medium supplemented with TA to enumerate spores. An untreated culture was plated onto rich medium to enumerate vegetative cells. Sporulation frequency was calculated by [CFUs from spores / total cell CFUs (spores + vegetative cells)] *100. C) Spores were exposed to UV for 10 minutes with constant agitation. After treatment, they were serially diluted and plated onto rich medium supplemented with TA. The ratio of treated to untreated CFUs of the mutant strains was then compared to the ratio from WT. pEV indicates an empty plasmid within the strain. All data points represent the average of three independent experiments. Statistical analysis by one-way ANOVA with Šίdák’s multiple comparisons test. * P<0.01, ** P<0.001, *** P<0.0001.