Figure 2. B. subtilis sspA complements C. difficile SASP mutant sporulation and UV defects.

A) Sporulation frequency of the indicated strain was determined as described in Figure 1. B) Spores were exposed to UV for 10 minutes with constant agitation. After treatment, they were serially diluted and plated onto rich medium supplemented with TA. The ratio of treated to untreated CFUs of the mutant strains was then compared to the ratio from WT. pEV indicates an empty plasmid within the strain. All data points represent the average of three independent experiments. Statistical analysis by A) one-way ANOVA with Tukey’s multiple comparisons test and B) unpaired T-test. * P<0.05, ** P<0.001, *** P<0.0001.