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[Preprint]. 2024 Jun 25:2023.05.17.541253. Originally published 2023 May 18. [Version 2] doi: 10.1101/2023.05.17.541253

Figure 4. Mutations in spoIVB2 suppress the C. difficile sspA sspB mutant phenotype.

Figure 4.

Sporulation was completed over 48 hours and the spore yield was determined. A) Sporulation of indicated strains with an empty vector (pEV) or wild type spoIVB2 B) Sporulation of C. difficile R20291 with the indicated spoIVB2 allele expressed from a plasmid. C) Sporulation assay of C. difficile R20291 ΔsspB* with the indicated spoIVB2 allele expressed from a plasmid. D) Sporulation assay of R20291 ΔsspA ΔsspB with the indicated spoIVB2 allele expressed from a plasmid. All data points represent the average from at least three independent experiments. Statistical analysis by two-way ANOVA with Šίdák’s multiple comparisons test* P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001.