Figure 7. Manipulation of spoIVB2_{F36 / F37} restores sporulation.

Spore yield of the indicated strain was determined as described in Figure 1. The plasmids expressing the spoIVB2_F37 alleles and the spoIVB2_F36F allele were assessed in the strains A) C. difficile ΔsspB*, B) C. difficile ΔsspA ΔsspB, C) C. difficile ΔspoIVB2. pEV indicates an empty plasmid within the strain. All data represents the average of five independent experiments. Statistical analysis by ANOVA with Šίdák’s multiple comparison test. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001.