Figure 9. Alternative promoters driving spoIVB2 rescues the sporulation phenotype.

Plasmids expressing spoIVB2 under various promoters were expressed in either wild type, the C. difficile ΔspoIVB2, or the C. difficile ΔsspA ΔsspB strains. Spore yield was determined after 48 hours of incubation by treatment with 30% EtOH and plating on medium containing germinants. The CFUs were log10 transformed. All data represents the average of three independent experiments. Statistical analysis by ANOVA with Šίdák’s multiple comparison test. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001.