|
Antibodies
|
| Rabbit polyclonal Anti-BabA sera |
Alexej Schmidt |
This study |
| Goat Anti-Rabbit Immunoglobulins/HRP |
DAKO |
Cat# P044801-2 |
| Goat Anti-Mouse Immunoglobulins/HRP |
DAKO |
Cat# P044701-2 |
| Human IgG, HRP-linked whole Ab (from sheep) |
Amersham ECL |
Cat# NA933-1ML |
| Biotin Anti-Myc tag antibody [9E10] |
Abcam |
Cat# ab81658 |
|
Bacterial strains
|
|
Helicobacter pylori CCUG17875 |
Culture Collection University of Gothenburg |
Cat# 17875 |
|
Helicobacter pylori CCUG17874 |
Culture Collection University of Gothenburg |
Cat# 17874 |
|
Chemicals
|
| BSA |
Sigma |
Cat# A7030 |
| EDTA |
Thermo Fisher Scientific |
Cat# AM9261 |
| Manganese (II) chloride |
Sigma |
Cat# 244589-10G |
| DAPI |
Thermo Fisher Scientific |
Cat# 62248 |
| Urea |
Sigma |
Cat# U0631-1KG |
| Trypsin |
Promega |
Cat# V5111 |
| Acetonitrile (ACN) |
Thermo Fisher Scientific |
Cat# A9554 |
| DNase I |
Sigma |
Cat# 4716728001 |
| b-mercaptoethanol |
Gibco |
Cat# 21985 |
| FITC |
Sigma |
Cat # 46950-250MG-F |
| Mayers HTX |
Histolab |
Cat# 01825 |
| Eosin Y 0,2% |
Histolab |
Cat# 01650 |
| Formaldehyde 4% Buffered |
Histolab |
Cat# 02178 |
| Lewis b HSA; Lacto-N-difucohexaose I-APD, HAS conjugate |
IsoSep AB |
Cas# 61/08-0005 |
| Dithiothreitol |
Sigma |
Cat# 10197777001 |
|
Critical commercial assays
|
| Pierce BCA Protein Assay Kit |
Thermo Fisher Scientific |
Cat# 23225 |
| Halt Protease Inhibitor Cocktail, EDTA-Free |
Thermo Fisher Scientific |
Cat# 78439 |
| LabSafe GEL Blue |
G-biosciences |
Cat# 786-35 |
| 7.5% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µL |
Bio-Rad |
Cat# 4561023 |
| HiTrap Protein G High Performance column |
Cytiva |
Cat# 17040401 |
| Disposable PD-10 Desalting Columns |
Cytiva |
Cat# 11768488 |
| Immun-Blot® PVDF Membrane |
Bio-Rad |
Cat# 1620177 |
| SuperSignal™ West Pico PLUS Chemiluminescent Substrate |
Thermo Fisher Scientific |
Cat# 34577 |
| Nunc™ 96-Well Polystyrene Round Bottom Microwell Plates |
Thermo Fisher Scientific |
Cat# 262162 |
