A) Representative images of the ligated hindlimb at each assayed time point as a function of treatment.
B) Modified ischemia scores (0–7 scale) at each assayed time point as a function of treatment. Each data point represents an individual mouse and data are presented as mean ± SD from N=5–9 biological replicates per condition. A two-way ANOVA mixed effects model with the Geisser-Greenhouse correction and Dunnett’s multiple comparison test was used to assess statistical significance (*, p<0.05; ***, p<0.001; ****, p<0.0001).
C) Tarlov mobility scores (0–6 scale) at each assayed time point as a function of treatment. Each data point represents an individual mouse and data are presented as mean ± SD from N=5–9 biological replicates per condition. A two-way ANOVA mixed effects model with the Geisser-Greenhouse correction and Dunnett’s multiple comparison test was used to assess statistical significance (*, p<0.05; **, p<0.01; ***, p<0.001).