| Name | Volume | Example Catalog |
|---|---|---|
| DMEM-F12 | 250 mL DMEM (+ 4.5 g/L D-glucose, + L-Glut, - Sodium Pyruvate) + 250 mL F-12 (+L-Glut) | Gibco 11965-092 Gibco 11765-054 |
| FBS Note: Do not heat inactivate FBS. Just thaw, swirl to mix, and go. |
129 mL | Atlanta Biologicals S11550 |
| Penicillin-Streptomycin | 6.4 mL | Gibco 15140 |
| Fungizone | 2.0 mL | Gibco 15290-018 |
| MEM Non-essential Amino Acids | 6.4 mL | Gibco 11140 |
| Beta-Mercaptoethanol | 6.4 μL | Gibco 21985-023 |
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