| Name | Volume |
|---|---|
| DMEM-F12 | 250 mL DMEM (+ 4.5 g/L D-glucose, + L-Glut, − Sodium Pyruvate) + 250 mL F-12 (+L-Glut) |
| FBS Note: Do not heat inactivate FBS. Just thaw, swirl to mix, and go. |
129 mL |
| Penicillin-Streptomycin | 6.4 mL |
| Fungizone | 2.0 mL |
| MEM Non-essential Amino Acids | 6.4 mL |
| Beta-Mercaptoethanol | 6.4 μL |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.